Biotechnology Engineering Interview Questions and Answers
Question - 61 : - Name some autoimmune diseases.
Answer - 61 : -
Rheumatoid arthritis, systemic lupus erythematosus, good pasture’s syndrome
Question - 62 : - How is the copy number of plasmid vector and yield of the recombinant protein related to each other?
Answer - 62 : - The copy number of plasmid vector is directly related to the yield of recombinant protein. Higher the copy number of vector plasmid, greater is the copy number of gene and consequently, the yield of the recombinant protein is in higher amounts.
Question - 63 : - Can exonuclease be used while producing a recombinant DNA molecule?
Answer - 63 : - No, an exonuclease cannot be used while producing a recombinant DNA. This is because an exonuclease degrades the DNA. It cannot produce DNA fragments with sticky ends.
Question - 64 : - What are the features of a plasmid being used as a cloning vector?
Answer - 64 : -
The characteristics of plasmids being used as a cloning vector are:
- It should have an origin of replication
- It should have a selectable marker
- It should have restriction sites
Answer - 65 : - Competent cells are those that allow the foreign DNA to incorporate into the host by a slight alteration in the cell walls. “Competent” means the ability of a cell to intake foreign DNA.
Question - 66 : - What do “Eco”, “R” and “I” refer to in the enzyme EcoRI?
Answer - 66 : - “Eco” refers to the species from which it is taken, “R” refers to the particular strain, and “I” states that it was the first enzyme isolated from this strain.
Question - 67 : - cWhy are proteases added while isolating the DNA?
Answer - 67 : - Proteases are added to degrade the proteins so that they do not interfere with the downstream DNA treatment.
Question - 68 : - If the “denaturation” step is missed during PCR, what would be its effect on the entire process?
Answer - 68 : - If the denaturation process is missed, there will no separation of DNA strands, the primers will not anneal to the template strands and eventually, amplification of DNA will not occur.
Question - 69 : - Name a recombinant vaccine.
Answer - 69 : - Hepatitis B vaccine is a recombinant vaccine.
Question - 70 : - Do biomolecules such as DNA, proteins exhibit biological activity in anhydrous conditions?
Answer - 70 : - No, biomolecules do not exhibit biological activity in anhydrous conditions. That is why life does not sustain without water.